Fig. 4. Western blotting of whole kidney homogenates. WT and KO mice were treated under control or acid-loading for 1-week and kidneys harvested (n = 5 or 6/group). Representative western blots and densitometry summary for males- A-B. rate-limiting gluconeogenic enzymes; C-D. PT transporters/exchangers; E-F. transcription factors and a prominent deacetylase. G. SIRT1 mRNA. Western blotting were normalized by Ponceau staining of membranes and the WTC mean was set to 1.0. Two-way ANOVA p-values are provided in graph inserts; *indicates a significant difference (p<0.05) from WT (within a treatment) by Tukey's multiple comparisons testing following a significant two-way ANOVA. WTC- wildtype control- closed circles; KOC- knockout control- open circles; WTA- wildtype acid- closed triangles; KOA- knockout acid- open triangles.